SubtiBank SubtiBank
fruA [2018-02-13T06:26:21.000Z]
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fruA [2018-02-13T06:26:21.000Z]

fructose-specific phosphotransferase system, EIIABC of the PTS
Locus
BSU14400
Isoelectric point
5.24
Molecular weight
67.01 kDa
Protein length
635 aa Sequence Blast
Gene length
1905 bp Sequence Blast
Function
fructose uptake and phosphorylation
Product
fructose-specific phosphotransferase system, EIIABC
Essential
no
E.C.
2.7.1.69
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,509,256 → 1,511,163

The protein

Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family

  • PtsI permease, fructose/ mannitol permease (Fru) family PubMed
  • Paralogous protein(s)

    Structure

  • 2R4Q (EIIB domain)
  • Localization

  • cell membrane (homogeneous) PubMed
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • FruR: repression, (according to DBTBS), in FruR regulon
  • Regulation

  • induced in the presence of fructose (FruR) (according to DBTBS)
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    Biological materials

    Mutant

  • BKE14400 (ΔfruA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
  • BKK14400 (ΔfruA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
  • References

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