SubtiBank SubtiBank
fruA [2018-02-13T06:26:21.000Z]
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fruA [2018-02-13T06:26:21.000Z]

fructose-specific phosphotransferase system, EIIABC of the PTS
locus
BSU14400
pI
5.24
mw
67.01 kDa
protein length
635 aa Sequence Blast
gene length
1905 bp Sequence Blast
function
fructose uptake and phosphorylation
product
fructose-specific phosphotransferase system, EIIABC
essential
no
ec
2.7.1.69
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.7|Utilization of fructose]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    1,509,256 → 1,511,163

    The protein

    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

    • Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, fructose/ mannitol permease (Fru) family [Pubmed|10627040]

    • Paralogous protein(s)

  • [protein|575CEC5C5C0458DC86A74F24654AC6990CB1E732|ManP]

    • Structure

  • [PDB|2R4Q] (EIIB domain)

    • [SW|Localization]

  • cell membrane (homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|fruR]-[gene|502AA809C8C95F8886DD91FBB7DDBECC63342327|fruK]-[gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|FruR]: repression, (according to [http://dbtbs.hgc.jp/COG/prom/fruRKA.html DBTBS]), in [regulon|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|FruR regulon]

    • regulation

  • induced in the presence of fructose ([protein|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|FruR]) (according to [http://dbtbs.hgc.jp/COG/prom/fruRKA.html DBTBS])
    • view in new tab

    Biological materials

    Mutant

  • BKE14400 (Δ[gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
  • BKK14400 (Δ[gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG

    • References

  • 16479537,10627040,21630458